Imaging Intracellular Fluorescent Proteins at Nanometer Resolution.

by: Eric Betzig, George H H Patterson, Rachid Sougrat, O Wolf W Lindwasser, Scott Olenych, Juan S S Bonifacino, Michael W W Davidson, Jennifer Lippincott-Schwartz, Harald F F Hess

Science (10 August 2006)

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A method for optically imaging intracellular proteins at nanometer spatial resolution is introduced. Numerous sparse subsets of photoactivatable fluorescent protein molecules are activated, localized (to ~2-25 nm), and then bleached. The aggregate position information from all subsets is then assembled into a superresolution image. The method, termed photoactivated localization microscopy (PALM), is demonstrated in thin sections by imaging specific target proteins in lysosomes and mitochondria, and in fixed, whole cells by imaging vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.

@article{citeulike:838625, abstract = {A method for optically imaging intracellular proteins at nanometer spatial resolution is introduced. Numerous sparse subsets of photoactivatable fluorescent protein molecules are activated, localized (to \~{}2-25 nm), and then bleached. The aggregate position information from all subsets is then assembled into a superresolution image. The method, termed photoactivated localization microscopy (PALM), is demonstrated in thin sections by imaging specific target proteins in lysosomes and mitochondria, and in fixed, whole cells by imaging vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.}, address = {Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA; New Millennium Research LLC, Okemos, MI 48864, USA.}, author = {Betzig, Eric and Patterson, George H H. and Sougrat, Rachid and Lindwasser, O Wolf W. and Olenych, Scott and Bonifacino, Juan S S. and Davidson, Michael W W. and Lippincott-Schwartz, Jennifer and Hess, Harald F F. }, citeulike-article-id = {838625}, doi = {http://dx.doi.org/10.1126/science.1127344}, issn = {1095-9203}, journal = {Science}, keywords = {super-resolution}, month = {August}, posted-at = {2008-07-15 01:34:27}, priority = {2}, title = {Imaging Intracellular Fluorescent Proteins at Nanometer Resolution.}, url = {http://dx.doi.org/10.1126/science.1127344}, year = {2006} }

RIS record

TY - JOUR ID - citeulike:838625 L3 - citeulike-article-id:838625 TI - Imaging Intracellular Fluorescent Proteins at Nanometer Resolution. JF - Science AD - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA; New Millennium Research LLC, Okemos, MI 48864, USA. SN - 1095-9203 N2 - A method for optically imaging intracellular proteins at nanometer spatial resolution is introduced. Numerous sparse subsets of photoactivatable fluorescent protein molecules are activated, localized (to ~2-25 nm), and then bleached. The aggregate position information from all subsets is then assembled into a superresolution image. The method, termed photoactivated localization microscopy (PALM), is demonstrated in thin sections by imaging specific target proteins in lysosomes and mitochondria, and in fixed, whole cells by imaging vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane. KW - super-resolution AU - Betzig, Eric AU - Patterson, George H AU - Sougrat, Rachid AU - Lindwasser, O Wolf AU - Olenych, Scott AU - Bonifacino, Juan S AU - Davidson, Michael W AU - Lippincott-Schwartz, Jennifer AU - Hess, Harald F PY - 2006/08/10/ UR - http://dx.doi.org/10.1126/science.1127344 ER -

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