An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP.

@article{citeulike:2932819, abstract = {Activity-regulated gene expression is believed to play a key role in the development and refinement of neuronal circuitry. Nevertheless, the transcriptional networks that regulate synapse growth and plasticity remain largely uncharacterized. Here, we show that microRNA 132 (miR132) is an activity-dependent rapid response gene regulated by the cAMP response element-binding (CREB) protein pathway. Introduction of miR132 into hippocampal neurons enhanced dendrite morphogenesis whereas inhibition of miR132 by 2'O-methyl RNA antagonists blocked these effects. Furthermore, neuronal activity inhibited translation of p250GAP, a miR132 target, and siRNA-mediated knockdown of p250GAP mimicked miR132-induced dendrite growth. Experiments using dominant-interfering mutants suggested that Rac signaling is downstream of miR132 and p250GAP. We propose that the miR132-p250GAP pathway plays a key role in activity-dependent structural and functional plasticity.}, address = {Department of Veterinary Anatomy, Physiology, and Pharmacology, Washington State University, 100 Dairy Road, Pullman, WA 99164;}, author = {Wayman, Gary A A. and Davare, Monika and Ando, Hideaki and Fortin, Dale and Varlamova, Olga and Cheng, Hai-Ying M Y. and Marks, Daniel and Obrietan, Karl and Soderling, Thomas R R. and Goodman, Richard H H. and Impey, Soren }, citeulike-article-id = {2932819}, doi = {http://dx.doi.org/10.1073/pnas.0803072105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {microrna, mir-132, p250gap}, month = {June}, posted-at = {2008-06-27 08:27:44}, priority = {2}, title = {An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP.}, url = {http://dx.doi.org/10.1073/pnas.0803072105}, year = {2008} }

TY - JOUR ID - citeulike:2932819 L3 - citeulike-article-id:2932819 TI - An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP. JF - Proceedings of the National Academy of Sciences of the United States of America AD - Department of Veterinary Anatomy, Physiology, and Pharmacology, Washington State University, 100 Dairy Road, Pullman, WA 99164; SN - 1091-6490 N2 - Activity-regulated gene expression is believed to play a key role in the development and refinement of neuronal circuitry. Nevertheless, the transcriptional networks that regulate synapse growth and plasticity remain largely uncharacterized. Here, we show that microRNA 132 (miR132) is an activity-dependent rapid response gene regulated by the cAMP response element-binding (CREB) protein pathway. Introduction of miR132 into hippocampal neurons enhanced dendrite morphogenesis whereas inhibition of miR132 by 2'O-methyl RNA antagonists blocked these effects. Furthermore, neuronal activity inhibited translation of p250GAP, a miR132 target, and siRNA-mediated knockdown of p250GAP mimicked miR132-induced dendrite growth. Experiments using dominant-interfering mutants suggested that Rac signaling is downstream of miR132 and p250GAP. We propose that the miR132-p250GAP pathway plays a key role in activity-dependent structural and functional plasticity. KW - microrna KW - mir-132 KW - p250gap AU - Wayman, Gary A AU - Davare, Monika AU - Ando, Hideaki AU - Fortin, Dale AU - Varlamova, Olga AU - Cheng, Hai-Ying M AU - Marks, Daniel AU - Obrietan, Karl AU - Soderling, Thomas R AU - Goodman, Richard H AU - Impey, Soren PY - 2008/06/24/ UR - http://dx.doi.org/10.1073/pnas.0803072105 ER -