Experimental and computational approaches to quantitative proteomics: Status quo and outlook.

@article{citeulike:2955666, abstract = {Proteomics has come a long way from the initial qualitative analysis of proteins present in a given sample at a given time ("cataloguing") to large-scale characterization of proteomes, their interactions and dynamic behavior. Originally enabled by breakthroughs in protein separation and visualization (by two-dimensional gels) and protein identification (by mass spectrometry), the discipline now encompasses a large body of protein and peptide separation, labeling, detection and sequencing tools supported by computational data processing. The decisive mass spectrometric developments and most recent instrumentation news are briefly mentioned accompanied by a short review of gel and chromatographic techniques for protein/peptide separation, depletion and enrichment. Special emphasis is placed on quantification techniques: gel-based, and label-free techniques are briefly discussed whereas stable-isotope coding and internal peptide standards are extensively reviewed. Another special chapter is dedicated to software and computing tools for proteomic data processing and validation. A short assessment of the status quo and recommendations for future developments round up this journey through quantitative proteomics.}, address = {Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, CH-1015 Lausanne, Switzerland; Functional Genomics Group, Department of Bioanalytical Science, Nestl\'{e} Research Centre, Vers-chez-les-Blanc, P.O. Box 44, CH-1000 Lausanne 26, Switzerland.}, author = {Panchaud, Alexandre and Affolter, Michael and Moreillon, Philippe and Kussmann, Martin }, citeulike-article-id = {2955666}, doi = {http://dx.doi.org/10.1016/j.jprot.2007.12.001}, issn = {1874-3919}, journal = {Journal of proteomics}, keywords = {proteomics, quantitative}, month = {April}, number = {1}, pages = {19--33}, posted-at = {2008-07-03 09:31:42}, priority = {2}, title = {Experimental and computational approaches to quantitative proteomics: Status quo and outlook.}, url = {http://dx.doi.org/10.1016/j.jprot.2007.12.001}, volume = {71}, year = {2008} }

TY - JOUR ID - citeulike:2955666 L3 - citeulike-article-id:2955666 TI - Experimental and computational approaches to quantitative proteomics: Status quo and outlook. JF - Journal of proteomics VL - 71 IS - 1 SP - 19 EP - 33 AD - Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, CH-1015 Lausanne, Switzerland; Functional Genomics Group, Department of Bioanalytical Science, Nestlé Research Centre, Vers-chez-les-Blanc, P.O. Box 44, CH-1000 Lausanne 26, Switzerland. SN - 1874-3919 N2 - Proteomics has come a long way from the initial qualitative analysis of proteins present in a given sample at a given time ("cataloguing") to large-scale characterization of proteomes, their interactions and dynamic behavior. Originally enabled by breakthroughs in protein separation and visualization (by two-dimensional gels) and protein identification (by mass spectrometry), the discipline now encompasses a large body of protein and peptide separation, labeling, detection and sequencing tools supported by computational data processing. The decisive mass spectrometric developments and most recent instrumentation news are briefly mentioned accompanied by a short review of gel and chromatographic techniques for protein/peptide separation, depletion and enrichment. Special emphasis is placed on quantification techniques: gel-based, and label-free techniques are briefly discussed whereas stable-isotope coding and internal peptide standards are extensively reviewed. Another special chapter is dedicated to software and computing tools for proteomic data processing and validation. A short assessment of the status quo and recommendations for future developments round up this journey through quantitative proteomics. KW - proteomics KW - quantitative AU - Panchaud, Alexandre AU - Affolter, Michael AU - Moreillon, Philippe AU - Kussmann, Martin PY - 2008/04/30/ UR - http://dx.doi.org/10.1016/j.jprot.2007.12.001 ER -