Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms.

@article{citeulike:2951469, abstract = {High-throughput, cell-based assays require small sample volumes to reduce assay costs and to allow for rapid sample manipulation. However, further miniaturization of conventional microtiter plate technology is problematic due to evaporation and capillary action. To overcome these limitations, we describe droplet-based microfluidic platforms in which cells are grown in aqueous microcompartments separated by an inert perfluorocarbon carrier oil. Synthesis of biocompatible surfactants and identification of gas-permeable storage systems allowed human cells, and even a multicellular organism (C. elegans), to survive and proliferate within the microcompartments for several days. Microcompartments containing single cells could be reinjected into a microfluidic device after incubation to measure expression of a reporter gene. This should open the way for high-throughput, cell-based screening that can use >1000-fold smaller assay volumes and has approximately 500x higher throughput than conventional microtiter plate assays.}, address = {Institut de Science et d'Ing\'{e}nierie Supramol\'{e}culaires, Universit\'{e} Louis Pasteur, Strasbourg Cedex, France.}, author = {Clausell-Tormos, J. and Lieber, D. and Baret, J. C. and El-Harrak, A. and Miller, O. J. and Frenz, L. and Blouwolff, J. and Humphry, K. J. and K\"{o}ster, S. and Duan, H. and Holtze, C. and Weitz, D. A. and Griffiths, A. D. and Merten, C. A. }, citeulike-article-id = {2951469}, doi = {http://dx.doi.org/10.1016/j.chembiol.2008.04.004}, issn = {1074-5521}, journal = {Chemistry \& biology}, keywords = {microfluidics}, month = {May}, number = {5}, pages = {427--437}, posted-at = {2008-07-02 09:02:47}, priority = {2}, title = {Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms.}, url = {http://dx.doi.org/10.1016/j.chembiol.2008.04.004}, volume = {15}, year = {2008} }

TY - JOUR ID - citeulike:2951469 L3 - citeulike-article-id:2951469 TI - Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms. JF - Chemistry & biology VL - 15 IS - 5 SP - 427 EP - 437 AD - Institut de Science et d'Ingénierie Supramoléculaires, Université Louis Pasteur, Strasbourg Cedex, France. SN - 1074-5521 N2 - High-throughput, cell-based assays require small sample volumes to reduce assay costs and to allow for rapid sample manipulation. However, further miniaturization of conventional microtiter plate technology is problematic due to evaporation and capillary action. To overcome these limitations, we describe droplet-based microfluidic platforms in which cells are grown in aqueous microcompartments separated by an inert perfluorocarbon carrier oil. Synthesis of biocompatible surfactants and identification of gas-permeable storage systems allowed human cells, and even a multicellular organism (C. elegans), to survive and proliferate within the microcompartments for several days. Microcompartments containing single cells could be reinjected into a microfluidic device after incubation to measure expression of a reporter gene. This should open the way for high-throughput, cell-based screening that can use >1000-fold smaller assay volumes and has approximately 500x higher throughput than conventional microtiter plate assays. KW - microfluidics AU - Clausell-Tormos, J AU - Lieber, D AU - Baret, JC AU - El-Harrak, A AU - Miller, OJ AU - Frenz, L AU - Blouwolff, J AU - Humphry, KJ AU - Köster, S AU - Duan, H AU - Holtze, C AU - Weitz, DA AU - Griffiths, AD AU - Merten, CA PY - 2008/05// UR - http://dx.doi.org/10.1016/j.chembiol.2008.04.004 ER -