Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.

@article{citeulike:2284554, abstract = {Despite the success of tyrosine kinase-based cancer therapeutics, for most solid tumors the tyrosine kinases that drive disease remain unknown, limiting our ability to identify drug targets and predict response. Here we present the first large-scale survey of tyrosine kinase activity in lung cancer. Using a phosphoproteomic approach, we characterize tyrosine kinase signaling across 41 non-small cell lung cancer (NSCLC) cell lines and over 150 NSCLC tumors. Profiles of phosphotyrosine signaling are generated and analyzed to identify known oncogenic kinases such as EGFR and c-Met as well as novel ALK and ROS fusion proteins. Other activated tyrosine kinases such as PDGFRalpha and DDR1 not previously implicated in the genesis of NSCLC are also identified. By focusing on activated cell circuitry, the approach outlined here provides insight into cancer biology not available at the chromosomal and transcriptional levels and can be applied broadly across all human cancers.}, address = {Cell Signaling Technology, 3 Trask Lane, Danvers, MA 01923, USA.}, author = {Rikova, K. and Guo, A. and Zeng, Q. and Possemato, A. and Yu, J. and Haack, H. and Nardone, J. and Lee, K. and Reeves, C. and Li, Y. and Hu, Y. and Tan, Z. and Stokes, M. and Sullivan, L. and Mitchell, J. and Wetzel, R. and Macneill, J. and Ren, J. M. and Yuan, J. and Bakalarski, C. E. and Villen, J. and Kornhauser, J. M. and Smith, B. and Li, D. and Zhou, X. and Gygi, S. P. and Gu, T. L. and Polakiewicz, R. D. and Rush, J. and Comb, M. J. }, citeulike-article-id = {2284554}, doi = {10.1016/j.cell.2007.11.025}, issn = {0092-8674}, journal = {Cell}, month = {December}, number = {6}, pages = {1190--1203}, posted-at = {2008-05-17 13:19:51}, priority = {2}, title = {Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.}, url = {http://dx.doi.org/10.1016/j.cell.2007.11.025}, volume = {131}, year = {2007} }

TY - JOUR ID - citeulike:2284554 L3 - citeulike-article-id:2284554 TI - Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. JF - Cell VL - 131 IS - 6 SP - 1190 EP - 1203 AD - Cell Signaling Technology, 3 Trask Lane, Danvers, MA 01923, USA. SN - 0092-8674 N2 - Despite the success of tyrosine kinase-based cancer therapeutics, for most solid tumors the tyrosine kinases that drive disease remain unknown, limiting our ability to identify drug targets and predict response. Here we present the first large-scale survey of tyrosine kinase activity in lung cancer. Using a phosphoproteomic approach, we characterize tyrosine kinase signaling across 41 non-small cell lung cancer (NSCLC) cell lines and over 150 NSCLC tumors. Profiles of phosphotyrosine signaling are generated and analyzed to identify known oncogenic kinases such as EGFR and c-Met as well as novel ALK and ROS fusion proteins. Other activated tyrosine kinases such as PDGFRalpha and DDR1 not previously implicated in the genesis of NSCLC are also identified. By focusing on activated cell circuitry, the approach outlined here provides insight into cancer biology not available at the chromosomal and transcriptional levels and can be applied broadly across all human cancers. AU - Rikova, K AU - Guo, A AU - Zeng, Q AU - Possemato, A AU - Yu, J AU - Haack, H AU - Nardone, J AU - Lee, K AU - Reeves, C AU - Li, Y AU - Hu, Y AU - Tan, Z AU - Stokes, M AU - Sullivan, L AU - Mitchell, J AU - Wetzel, R AU - Macneill, J AU - Ren, JM AU - Yuan, J AU - Bakalarski, CE AU - Villen, J AU - Kornhauser, JM AU - Smith, B AU - Li, D AU - Zhou, X AU - Gygi, SP AU - Gu, TL AU - Polakiewicz, RD AU - Rush, J AU - Comb, MJ PY - 2007/12/14/ UR - http://dx.doi.org/10.1016/j.cell.2007.11.025 ER -