A proteomic analysis of cell signaling alterations in colorectal cancer

@article{citeulike:1893069, abstract = {To gain further insight into alterations in cellular pathways, tumor profiling and marker discovery in colorectal cancer (CRC) we have used a new antibody microarray specific for cell signaling. Soluble protein extracts were prepared from paired tumor/normal biopsies of 11 patients diagnosed of colorectal carcinoma at different stages, four liver carcinomas were used as a reference. Antibody microarray analysis identified 46 proteins that were differentially expressed between normal colorectal epithelium and adenocarcinoma. These proteins gave a specific signature for CRC, different from other tumors, as well as a panel of novel markers and potential targets for CRC. Twenty-four proteins were validated by using a specific colorectal cancer tissue microarray and immunoblotting analysis. Together with some previously well known deregulated proteins in CRC (-catenin, c-myc, or p63), we have found new potential markers preferentially expressed in CRC tumors: cytokeratin 13, calcineurin, Chk1, clathrin light chain, MAPK3, phospho-PTK2/FAK (S910) and MDM2. Chk1 antibodies were particularly effective in discriminating between tumoral and normal mucosa in CRC. Moreover, a global picture of alterations in signaling pathways in CRC was observed, including a significant up-regulation of different components of the EGFR and Wnt/-catenin pathways and the down-regulation of p14ARF. The experimental approach described here should be applicable to other pathologies and neoplasic processes. 10.1074/mcp.M700006-MCP200}, author = {Madoz-Gurpide, Juan and Canamero, Marta and Sanchez, Lydia and Solano, Jose and Alfonso, Patricia and Casal, Ignacio J. }, citeulike-article-id = {1893069}, doi = {http://dx.doi.org/10.1074/mcp.M700006-MCP200}, journal = {Mol Cell Proteomics}, keywords = {cancer, pathways, signaling}, month = {September}, pages = {M700006-MCP200+}, posted-at = {2007-11-10 04:02:34}, priority = {3}, title = {A proteomic analysis of cell signaling alterations in colorectal cancer}, url = {http://dx.doi.org/10.1074/mcp.M700006-MCP200}, year = {2007} }

TY - JOUR ID - citeulike:1893069 L3 - citeulike-article-id:1893069 TI - A proteomic analysis of cell signaling alterations in colorectal cancer JF - Mol Cell Proteomics SP - M700006-MCP200 N2 - To gain further insight into alterations in cellular pathways, tumor profiling and marker discovery in colorectal cancer (CRC) we have used a new antibody microarray specific for cell signaling. Soluble protein extracts were prepared from paired tumor/normal biopsies of 11 patients diagnosed of colorectal carcinoma at different stages, four liver carcinomas were used as a reference. Antibody microarray analysis identified 46 proteins that were differentially expressed between normal colorectal epithelium and adenocarcinoma. These proteins gave a specific signature for CRC, different from other tumors, as well as a panel of novel markers and potential targets for CRC. Twenty-four proteins were validated by using a specific colorectal cancer tissue microarray and immunoblotting analysis. Together with some previously well known deregulated proteins in CRC (-catenin, c-myc, or p63), we have found new potential markers preferentially expressed in CRC tumors: cytokeratin 13, calcineurin, Chk1, clathrin light chain, MAPK3, phospho-PTK2/FAK (S910) and MDM2. Chk1 antibodies were particularly effective in discriminating between tumoral and normal mucosa in CRC. Moreover, a global picture of alterations in signaling pathways in CRC was observed, including a significant up-regulation of different components of the EGFR and Wnt/-catenin pathways and the down-regulation of p14ARF. The experimental approach described here should be applicable to other pathologies and neoplasic processes. 10.1074/mcp.M700006-MCP200 KW - cancer KW - pathways KW - signaling AU - Madoz-Gurpide, Juan AU - Canamero, Marta AU - Sanchez, Lydia AU - Solano, Jose AU - Alfonso, Patricia AU - Casal, Ignacio PY - 2007/09/11/ UR - http://dx.doi.org/10.1074/mcp.M700006-MCP200 ER -