Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas

@article{citeulike:2924279, abstract = {Host-vector systems have been developed for gene cloning in the metabolically versatile bacterial genus Pseudomonas. They comprise restriction-negative host strains of Pseudomonas aeruginosa and P. putida and new cloning vectors derived from the high-copy-number, broad-host-range plasmid RSF1010, which are stably maintained in a wide range of Gram-negative bacteria. These plasmids contain EcoRI, SstI, HindIII, XmaI, XhoI, SalI, BamHI and ClaI insertion sites. All cloning sites, except for BamHI and ClaI, are located within antibiotic-resistance genes; insertional inactivation of these genes during hybrid plasmid formation provides a readily scored phenotypic change for the rapid identification of bacterial clones carrying such hybrids. One of the new vector plasmids is a cosmid that may be used for the selective cloning of large DNA fragments by in vitro [lambda] packaging. An analogous series of vectors that are defective in their plasmid-mobilization function, and that exhibit a degree of biological containment comparable to that of current Escherichia coli vector plasmids, are also described.}, author = {Bagdasarian, M. and Lurz, R. and R\"{u}ckert, B. and Franklin, F. C. H. and Bagdasarian, M. M. and Frey, J. and Timmis, K. N. }, citeulike-article-id = {2924279}, doi = {10.1016/0378-1119(81)90080-9}, journal = {Gene}, keywords = {kt2440, putida}, month = {December}, number = {1-3}, pages = {237--247}, posted-at = {2008-06-24 21:14:11}, priority = {2}, title = {Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas}, url = {http://dx.doi.org/10.1016/0378-1119(81)90080-9}, volume = {16}, year = {1981} }

TY - JOUR ID - citeulike:2924279 L3 - citeulike-article-id:2924279 TI - Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas JF - Gene VL - 16 IS - 1-3 SP - 237 EP - 247 N2 - Host-vector systems have been developed for gene cloning in the metabolically versatile bacterial genus Pseudomonas. They comprise restriction-negative host strains of Pseudomonas aeruginosa and P. putida and new cloning vectors derived from the high-copy-number, broad-host-range plasmid RSF1010, which are stably maintained in a wide range of Gram-negative bacteria. These plasmids contain EcoRI, SstI, HindIII, XmaI, XhoI, SalI, BamHI and ClaI insertion sites. All cloning sites, except for BamHI and ClaI, are located within antibiotic-resistance genes; insertional inactivation of these genes during hybrid plasmid formation provides a readily scored phenotypic change for the rapid identification of bacterial clones carrying such hybrids. One of the new vector plasmids is a cosmid that may be used for the selective cloning of large DNA fragments by in vitro [lambda] packaging. An analogous series of vectors that are defective in their plasmid-mobilization function, and that exhibit a degree of biological containment comparable to that of current Escherichia coli vector plasmids, are also described. KW - kt2440 KW - putida AU - Bagdasarian, M AU - Lurz, R AU - Rückert, B AU - Franklin, FCH AU - Bagdasarian, MM AU - Frey, J AU - Timmis, KN PY - 1981/12// UR - http://dx.doi.org/10.1016/0378-1119(81)90080-9 ER -