Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes

by: Michael E Kovach, Philip H Elzer, Steven, Gregory T Robertson, Michael A Farris, Martin R Roop, Kenneth M Peterson

Gene, Vol. 166, No. 1. (1 December 1995), pp. 175-176.

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Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed. These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small (< 5.3 kb), (ii) they possess an extended multiple cloning site (MCS), (iii) they allow direct selection of recombinant plasmid molecules in Escherichia coli via disruption of the LacZ[alpha] peptide, (iv) they are mobilizable when the RK2 transfer functions are provided in trans and (v) they are compatible with IncP, IncQ and IncW group plasmids, as well as with ColE1- and P15a-based replicons.

@article{citeulike:1219840, abstract = {Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed. These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small (< 5.3 kb), (ii) they possess an extended multiple cloning site (MCS), (iii) they allow direct selection of recombinant plasmid molecules in Escherichia coli via disruption of the LacZ[alpha] peptide, (iv) they are mobilizable when the RK2 transfer functions are provided in trans and (v) they are compatible with IncP, IncQ and IncW group plasmids, as well as with ColE1- and P15a-based replicons.}, author = {Kovach, Michael E. and Elzer, Philip H. and Steven and Robertson, Gregory T. and Farris, Michael A. and Roop, Martin R. and Peterson, Kenneth M. }, citeulike-article-id = {1219840}, doi = {10.1016/0378-1119(95)00584-1}, journal = {Gene}, keywords = {aeruginosa, plasmid}, month = {December}, number = {1}, pages = {175--176}, posted-at = {2008-06-27 11:13:07}, priority = {2}, title = {Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes}, url = {http://dx.doi.org/10.1016/0378-1119(95)00584-1}, volume = {166}, year = {1995} }

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TY - JOUR ID - citeulike:1219840 L3 - citeulike-article-id:1219840 TI - Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes JF - Gene VL - 166 IS - 1 SP - 175 EP - 176 N2 - Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed. These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small (< 5.3 kb), (ii) they possess an extended multiple cloning site (MCS), (iii) they allow direct selection of recombinant plasmid molecules in Escherichia coli via disruption of the LacZ[alpha] peptide, (iv) they are mobilizable when the RK2 transfer functions are provided in trans and (v) they are compatible with IncP, IncQ and IncW group plasmids, as well as with ColE1- and P15a-based replicons. KW - aeruginosa KW - plasmid AU - Kovach, Michael AU - Elzer, Philip AU - Steven AU - Robertson, Gregory AU - Farris, Michael AU - Roop, Martin AU - Peterson, Kenneth PY - 1995/12/01/ UR - http://dx.doi.org/10.1016/0378-1119(95)00584-1 ER -

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